Indicators of induced subacute ruminal acidosis (SARA) in Danish Holstein cows

Table 6 Results of ruminal pH and ruminal fluid metabolites

	Control diet	SARA diet	SED	P values
Ruminal pH
Mean	6.31	6.06	0.07	0.01
Minimum	5.79	5.36	0.07	0.001
Maximum	6.73	6.77	0.10	0.77
Minutes < pH 5.8	77.6	490.7	112.9	0.01
Minutes < pH 5.6	11.0	294.5	93.4	0.02
Area under the curve < pH 5.8	11.3	143.6	34.0	0.02
Area under the curve < pH 5.6	0	66.5	24.6	0.04
Volatile fatty acids (VFA)
Total (mM)	129.6	123.6	10.1	0.73
Acetate (mM)	81.9	69.0	6.4	0.02
Propionate (mM)	26.7	30.4	4.4	0.50
Butyrate (mM)	16.3	19.5	1.7	0.25
Valerate (mM)	1.89	2.27	0.44	0.42
Isovalerate (mM)	1.79	1.72	0.20	0.62
Isobutyrate (mM)	1.04	0.76	0.06	<.0001
Caproate (mM)	0.60	0.45	0.16	0.37
l-Lactate (mM)	0.19	0.28	0.15	0.57
Acetate:propionate	3.23	2.52	0.41	0.09

SED standard error of difference.
Ruminal pH parameters and concentration of ruminal fluid metabolites from cows fed regular TMR diet (control) and TMR + wheat-barley pellets (SARA). Estimates are given as least squares means from the repeated measurements model. Acetate, propionate, butyrate, valerate, isovalarete, and lactate concentrations were transformed back to the original scale.

ISSN: 1751-0147