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Fig. 1 | Acta Veterinaria Scandinavica

Fig. 1

From: Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining

Fig. 1

Gating strategy to quantify cytokine+ canine lymphocytes following short-term whole blood cultures in vitro. Upper panels (controls) and lower panels (stimulated with PMA + LPS). a A plot of spot size distribution (FSC) versus granularity (SSC) used for the selection of the lymphocyte population of interest—with the (R1) phenotype FSC × SSC. b Dot plot distribution of cytokine+ cells built by bi-dimensional graphs of empty channel (FL4 fluorescence) versus PE-emission channel (FL2) were used to quantify the percentage of cytokine+ lymphocytes within R1. The left part of the graph (Quadrants Q1 and Q4, highlighted by gray background) was used to compose the figures for subsequent analyses. c In the Q1 quadrant are shown the percentage (%) of cytokine+ lymphocytes using TNF-α as example

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Acta Veterinaria Scandinavica

ISSN: 1751-0147