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Fig. 3 | Acta Veterinaria Scandinavica

Fig. 3

From: Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining

Fig. 3

Internal controls to validate the cross-reactivity of anti-human cytokine mAbs with canine cytokines following short-term whole blood cultures in vitro. Unspecific binding were monitored by using isotypic matching reagents (top panels). Blocking strategy with host serum were also applied (bottom panels). Controls of reference cross-reactive mAbs (anti-bovina IFN-γ and IL-4) were also included. Dot plot distribution graphs of empty channel (FL4 fluorescence) versus PE-emission channel (FL2) were used to quantify the percentage of cytokine+ lymphocytes in unstimulated controls (left panels) and PMA + LPS stimulated cultures (right panels). Only Q1 and Q4 quadrants are represented. Gray background highlights the reactivity above 0.5 % in the quadrant Q1

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Acta Veterinaria Scandinavica

ISSN: 1751-0147