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Fig. 4 | Acta Veterinaria Scandinavica

Fig. 4

From: Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining

Fig. 4

Applicability of cross-reacted anti-human cytokine mAbs (IL-1-α, IL-6, IL-12, IL-17A and TGF-β) to quantify cytokine+ lymphocytes from unhealthy dogs, including peritonitis (Dog #1) and dermatitis (Dog #2). Dot plot distribution graphs of empty channel (FL4 fluorescence) versus PE-emission channel (FL2) were used to quantify the percentage of cytokine+ lymphocytes in unstimulated controls (left panels) and PMA + LPS stimulated cultures (right panels). Only Q1 and Q4 quadrants are represented. Gray background highlights the reactivity above 0.5 % in the quadrant Q1

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Acta Veterinaria Scandinavica

ISSN: 1751-0147