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Fig. 5 | Acta Veterinaria Scandinavica

Fig. 5

From: Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining

Fig. 5

Correlation analysis of cytokine+ lymphocytes detected by commercially available anti-human cytokine mAbs (IL-4 and IFN-γ) and the reference cross-reactivity reagent (anti-bonive IL-4 and IFN-γ). a Dot plot distribution graphs of empty channel (FL4 fluorescence) versus PE-emission channel (FL2) were used to quantify the percentage of cytokine+ lymphocytes (IFN-γ #1—upper panel and IL-4—bottom panel). Only Q1 and Q4 quadrants are represented. Gray background highlights the reactivity above 0.5 % in the quadrant Q1. b Spearman correlation plots of the frequency of cytokine+ lymphocytes stained with each mAbs (anti-human versus reference anti-bovine mAbs)

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Acta Veterinaria Scandinavica

ISSN: 1751-0147