Fig. 3
From: Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells
Intracellular localization of Brucella pinnipedialis hooded seal strain. Double immune labeling with anti-Brucella antibody (green) and anti-pan cytokeratin antibody (red) confirmed intracellular presence of hooded seal B. pinnipedialis strain B17 (arrowheads) in hooded seal epithelial cells. Scale bar 20 μm. The confocal image is a single optical section of 0.5 μm captured perpendicular to the Z-axis at the level of the nucleus using a Zeiss LSM510 META system (Carl Zeiss, Obercochen, Germany) equipped with a 40X 1.2NA water immersion lens