Table 4 Insulin concentrations measured in follicular fluid and serum based on published literature

0.21–0.48

–

A specific double antibody RIA

[13]

0.32–0.40

–

125I-labeled insulin

Double-antibody RIA

[14]

0.42–1.88

–

125I-labeled insulin

Double-antibody RIA

[15]

0.349–0.712

Luteal phase = 0.349; Follicular phase = 0.417;

Peak = 0.712;

Mean = 0.5–0.69

0.127–0.282

Subordinate = 0.12–0.17;

Preovulatory = 0.3–0.72

RIA Coat-A-Count kit (DPC, Los Angeles, CA, USA) validated for cattle by [16]

[17]

–

0.5–10

–

[11]

0.21–0.34

–

ELISA, validated for cattle (Immuno-Biological Laboratories, Hamburg, Germany)

[18]

–

0.38–0.42

By EIA not specified

[19]

–

0.59 (acyclic)

1.01 (cyclic)

RIA (RIAK-1 kit, BRIT, Navi Mumbai, India)

[20]

–

0.27 (cyst)

1.15 (normal)

RIA (RIAK-1 kit, BRIT, Navi Mumbai, India)

[21]

0.24 (heifer)

0.51 (cow)

–

Insulin auto DELFIA (PerkinElmer Life and Analytical Science, Turku, Finland)

[22]

0.4–0.6

–

EIA

[23]