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Table 1 In vitro proliferative responses (SI ± SD) of peripheral blood lymphocytes after stimulation with PHA, ConA mitogens and H5 antigens in mute swans from control and experimental groups (C, D1, D2)

From: The response of mute swans (Cygnus olor, Gm. 1789) to vaccination against avian influenza with an inactivated H5N2 vaccine

Mitogen concentrations [µg/ml] or antigen (HA titer)

WeekNS

SI ± SD values in groups of mute swansNS

C

D1

D2

PHA 5.0 µg/ml

0

1.29 ± 0.13

0.68 ± 0.78

1.31 ± 1.10

4

0.90 ± 0.06

1.26 ± 0.18

0.96 ± 0.13

8

1.22 ± 0.06

1.20 ± 0.36

1.20 ± 0.09

PHA 2.5 µg/ml

0

1.14 ± 0.13

0.77 ± 1.02

1.43 ± 0.33

4

1.16 ± 0.23

0.55 ± 0.14

0.90 ± 0.62

8

1.41 ± 0.09

1.73 ± 0.38

1.53 ± 0.16

PHA 1.25 µg/ml

0

1.59 ± 0.44

1.5 ± 0.58

1.88 ± 0.28

4

1.26 ± 0.36

1.01 ± 0.18

1.21 ± 0.74

8

1.17 ± 0.86

2.41 ± 1.49

1.09 ± 0.34

ConA 5.0 µg/ml

0

1.27 ± 0.18

1.46 ± 0.06

1.74 ± 0.59

4

0.92 ± 0.07

1.98 ± 1.02

1.21 ± 0.34

8

1.14 ± 0.11

1.26 ± 0.45

1.84 ± 0.51

ConA 2.5 µg/ml

0

1.44 ± 0.33

1.85 ± 0.46

1.67 ± 0.51

4

1.02 ± 0.16

2.18 ± 1.08

1.58 ± 0.91

8

1.70 ± 0.37

1.86 ± 0.63

3.16 ± 1.46

ConA 1.25 µg/ml

0

1.82 ± 0.26

1.41 ± 0.14

1.15 ± 0.48

4

1.06 ± 0.18

0.99 ± 0.64

1.29 ± 0.67

8

1.93 ± 0.87

1.69 ± 0.00

3.17 ± 1.51

H5 (HA 1/8)

0

0.96 ± 0.05

1.29 ± 0.09

0.80 ± 0.58

4

1.01 ± 0.07

0.56 ± 0.25

0.90 ± 0.00

8

1.24 ± 0.22

1.18 ± 0.42

1.2 ± 0.15

H5 (HA 1/16)

0

1.04 ± 0.38

0.83 ± 0.43

1.03 ± 0.46

4

0.77 ± 0.10

0.45 ± 0.20

0.79 ± 0.00

8

1.27 ± 0.26

1.60 ± 0.81

1.31 ± 0.11

  1. NS no statistically significant differences based on analysis of variance and Fisher’s multiple comparisons in SI between weeks and groups for a given mitogen