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Table 4 Reaction score to udder palpation (Reaction score), udder edema score (Edema score), udder temperature (Udder T) and mechanical nociceptive thresholds (MNT) measured in infused quarters of cows challenged with intramammary LPS (lipopolysaccharide), LTA (lipoteichoic acid) or saline (control group)

From: Experimentally induced subclinical mastitis: are lipopolysaccharide and lipoteichoic acid eliciting similar pain responses?

Parameter

Control day

Challenge day

2 h

4 h

6 h

8 h

2 h

4 h

6 h

8 h

Reaction score

 LPS

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

 LTA

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

 Control

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

Edema score

 LPS

0 (0–1)

0 (0–0)

0 (0–0)

0 (0–0)

1 (1–1)

2.5 (2–3)

2 (1–3)

1.5 (1–2)

 LTA

0 (0–1)

0 (0–1)

0 (0–1)

0 (0–1)

0 (0–0.2)

1 (0.75–2)

1 (0.75–2.25)

1 (0.75–1.5)

 Control

0 (0–0)

0 (0–0.5)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0)

0 (0–0.5)

1 (0.5–1)

Udder T (°C)

 LPS

36.3 (34.7–36.9)

36.6 (34.7–37.4)

36.2 (35–37)

36.9 (36.6–37.2)

36.1 (36–37.5)

36.4 (35.8–37)

36.8 (36–37.3)

36.4 (36–37.2)

 LTA

35.1 (34.1–36.6)

34.7 (34–37.4)

35.9 (34.3–37.1)

35.5 (33.8–37.4)

34.4 (33.6–36.8)

35.2 (34.1–37.0)

36.2 (34.3–37.7)

36.9 (33.9–37.6)

 Control

33.9 (32.5–35.1)

34 (32.8–35)

34.3 (32.9–35.7)

34.6 (33–35.8)

33.5 (32.9–34.8)

34.4 (33.3–35.5)

33.9 (33.1–35.2)

34.5 (33.5–36)

MNT (N)

 LPS

22.4 (13.4–24.6)

22 (11–24.6)

22.7 (11.8–24.6)

24.6 (22.6–24.6)

18.3 (13.9–23.4)

23.6 (18.2–24.6)

23.9 (19.1–24.6)

21.8 (15.8–24.6)

 LTA

24.6 (24.6–24.6)

23.3 (16.6–24.6)

24.6 (19.7–24.6)

24.6 (17.3–24.6)

24.6 (19.4–24.6)

24.6 (21.7–24.6)

23.9 (17.6–24.6)

18.2 (10.7–24.6)

 Control

24.6 (23.8–24.6)

24.6 (24.6–24.6)

20.8 (19.2–24.6)

24.6 (22–24.6)

15.5 (11.5–20.6)

22.1 (20.5–23.9)

18.2 (12–23.6)

20.4 (15.1–24)

  1. On the control day, the intramammary challenge, milk and blood sampling were simulated. Medians and interquartile ranges (IQR) at 2, 4, 6 and 8 h after the simulated challenge (control day)/challenge (challenge day) are reported