Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters

Table 2 Plasma membrane integrity, acrosome integrity, sperm chromatin structure assay and mitochondrial activity of boar spermatozoa in semen stored for 240 h at 17 °C (mean ± SD, n = 35)

Spermatozoa (%)	Hours of incubation					Source of variability
Spermatozoa (%)	0 h	48 h	96 h	168 h	240 h	Boar	Time	Interaction
Live (SYBR+ PI−)	88.8 ± 8.2^A	88.5 ± 6.6^A	88.1 ± 7.7^A	87.4 ± 7.9^A	85.5 ± 7.7^B	<0.0001	0.0433	0.9981
Live with intact acrosome (PNA− PI−)	82.5 ± 7.5^AB	85.0 ± 6.1^A	81.5 ± 9.1^AB	80.1 ± 8.9^B	78.6 ± 8.0^B	<0.0001	0.0005	0.9987
%HDS	0.82 ± 0.5	0.73 ± 0.3	0.65 ± 0.3	0.79 ± 0.3	0.84 ± 0.3	<0.0001	0.1407	0.8690
%DFI	3.55 ± 2.7^A	3.95 ± 2.5^B	4.14 ± 2.4^BC	4.40 ± 2.4^Ca	4.71 ± 2.2^Cb	<0.0001	0.0200	0.9996
Live with active mitochondria (R+ PI−)	83.0 ± 6.4	83.2 ± 6.9	82.2 ± 6.7	81.7 ± 7.2	79.1 ± 6.9	<0.0001	0.1188	0.9999

SYBR, SYBR-14; PI, propidium iodide; PNA, PNA Alexa Fluor 488 (lectin from Arachis hypogaea); PI, propidium iodide; %DFI (DNA fragmentation index)—the percentage of spermatozoa with DNA fragmentation; %HDS (high DNA stainability)—the percentage of spermatozoa with immature chromatin (less chromatin condensation); R, Rhodamine 123; PI, propidium iodide
Different superscripts within a row indicate significant differences ^a,b P < 0.05; ^A,B,C P < 0.01

ISSN: 1751-0147