Table 1 Immunohistochemistry panel: specificity, positive control, dilution and result for each antibody used for analyses
Primary antibody | Antibody specificity | Positive control | Dilution | Result |
|---|---|---|---|---|
Vimentin | Clone V9 (Invitrogen®) | Internal (mesenchymal cells; fibroblasts lion) | 1:2000/1500 | (+) |
S100 | Polyclonal (Dako®) | Human intestine and tiger intestine (neural plexus) | 1:5000 | (+) |
PLAP | Clone 8A9 (Dako®) | Human and tiger testicle (placental alkaline phosphatase in germ cells) | 1:200 | (−) |
Inhibin | Clone Bc/R1 (Biocare®) | Human and tiger testicle (stromal and sex cord cells) | 1:200 | NI |
Cytokeratin AE1 + AE3 | Clone Isotyp IgG1 (Biocare®) | Human intestine and tiger intestine (epithelium) | 1:1000 | (−) |
cKit/CD117 | Clone YR145 (Cell Marque®) | Skin metastasis of human gastrointestinal stromal tumour and lion skin (cutaneous mastocytes) | 1:500 | (−) |
Ki67 | Clone MIB-1 (Dako®) | Human tonsil (tonsillar epithelium) and tiger intestine (epithelium) | 1:100 | NI |