Fig. 4From: In vitro investigations on extracellular proteins secreted by Aphanomyces invadans, the causative agent of epizootic ulcerative syndromeProtease activity of Aphanomyces invadans secreted proteases against IgM of rainbow trout (RT) and giant gourami (GG). Spot 1: IgM was degraded by proteases as no dark spot is seen. Spot 2: IgM was intact when proteases were heat inactivated and upon addition of anti-antibodies and the substrate produced a dark spot. 3: ECP and IgM both were heat inactivated hence produced no dark spot. Spots 4 and 5: IgM was intact in GPY media and distilled water respectively hence produced dark spots. Spot 6: EDTA, Spot 7: E-64 protease inhibitor and Spot 8: phenylmethanesulfonyl fluoride inhibited the proteases hence dark spots are seenBack to article page