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Fig. 4 | Acta Veterinaria Scandinavica

Fig. 4

From: In vitro investigations on extracellular proteins secreted by Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome

Fig. 4

Protease activity of Aphanomyces invadans secreted proteases against IgM of rainbow trout (RT) and giant gourami (GG). Spot 1: IgM was degraded by proteases as no dark spot is seen. Spot 2: IgM was intact when proteases were heat inactivated and upon addition of anti-antibodies and the substrate produced a dark spot. 3: ECP and IgM both were heat inactivated hence produced no dark spot. Spots 4 and 5: IgM was intact in GPY media and distilled water respectively hence produced dark spots. Spot 6: EDTA, Spot 7: E-64 protease inhibitor and Spot 8: phenylmethanesulfonyl fluoride inhibited the proteases hence dark spots are seen

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