Quantitative method for analysis of six anticoagulant rodenticides in faeces, applied in a case with repeated samples from a dog

Table 1 Validation parameters of six anticoagulant rodenticides

Compound	Calibration range (ng/mL or ng/g)	Blood		Faeces		QC-sample conc.	Blood					Faeces
Compound	Calibration range (ng/mL or ng/g)	Mean R²	RSD (%)	Mean R²	RSD (%)	QC-sample conc.	Within-day precision RSD (%)	Between-day precision RSD (%)	Bias (%)	ME%	RE%	Within-day precision RSD (%)	Between-day precision RSD (%)	Bias (%)	ME%	RE%
Coumatetralyl	1.5–731 (0.0050–2.5 µM)	0.999	0.061	0.998	0.24	2.3 88 585	4.0 3.6 6.0	7.9 8.7 11	− 3.5 5.2 − 1.8	90 93	10 15	2.8 5.7 6.5	15 13 15	− 3.6 1.6 8.4	109 96	69
Bromadiolone	2.6–1319 (0.0050–2.5 µM)	0.998	0.21	0.998	1	4.2 158 1055	5.8 5.7 7.5	17 14 17	16 13 − 4.1	100 97	44 52	15 17 16	16 18 13	− 7.7 − 3.1 − 11	63 56	32
Difenacoum	2.2–1111 (0.0050–2.5 µM)	0.997	0.33	0.999	0.10	3.6 133 889	9.1 2.2 8.9	13 15 15	5.5 7.8 6.1	101 97	43 87	13 14 15	18 17 15	− 5.3 − 6.1 − 11	62 60	26
Flocoumafen	2.7–1356 (0.0050–2.5 µM)	0.997	0.41	0.995	1.1	4.3 163 1085	8.0 3.0 6.4	14 14 15	2.7 6.6 − 4.6	95 92	80 90	11 9.0 6.3	19 17 19	− 5.5 − 2.8 − 14	45 32	18
Brodifacoum	2.6–1309 (0.0050–2.5 µM)	0.998	0.37	0.999	0.11	4.2 157 1047	8.6 3.1 9.1	15 14 11	8.4 6.2 − 3.1	93 90	61 78	9.2 9.3 6.4	19 18 19	− 1.7 − 2.1 − 18	32 32	25
Difethialone	2.7–1349 (0.0050–2.5 µM)	0.997	0.37	0.998	0.17	4.3 164 1079	8.9 6.4 13	14 12 17	16 12 3.1	86 81	69 84	10 11 5.1	17 11 16	1.3 0.8 − 9.4	31 27	22

ISSN: 1751-0147