Prevalence and phylogenetic analysis of hepatitis E virus in pigs, wild boars, roe deer, red deer and moose in Lithuania

Table 1 Primer sets used in this study

Primer designation	Sequence (5ʹ → 3ʹ)	Step	Product length (bp)	Target region
HEV-s	TCGCGCATCACMTTYTTCCARAA	RT-PCR	469	ORF1
HEV-as	GCCATGTTCCAGACDGTRTTCCA	RT-PCR	469
HEV-fn	TGTTGCCCTGTTTGGCCCCTGGTTTAG	Nested RT-PCR	254
HEV-rn	CCAGGCTCACCRGARTGYTTCTTCCA	Nested RT-PCR	254
3156F	AATTATGCYCAGTAYCGRGTTG	RT-PCR	731	ORF2
3157R	CCCTTRTCYTGCTGMGCATTCTC	RT-PCR	731
3158Fn	GTWATGCTYTGCATWCATGGCT	Nested RT-PCR	348
3159Rn	AGCCGACGAAATCAATTCTGTC	Nested RT-PCR	348

ISSN: 1751-0147