Table 1 Study overview
From: A porcine model for pathomorphological age assessment of surgically excised skin wounds
Wound age | Pig | No. of wounds biopsied | No. of wounds sampled post-mortem | Histological features relevant for age determination | Average neutrophil score + SD | Median neutrophil score (range) | Average macrophage score + SD | Median macrophage score (range) | N:M ratio |
|---|---|---|---|---|---|---|---|---|---|
1 h | 1 | 4 | Clot | 4.3 ± 1.7 | 4.5 (2–6) | 1 ± 0 | 1 (1–1) | 4:1 | |
3 h | 2, 3 | 8 | 5.9 ± 0.4 | 6 (5–6) | 1.5 ± 0.5 | 1.5 (1–2) | 4:1 | ||
6 h | 4, 5 | 8 | 4.8 ± 0.9 | 5 (3–6) | 1.8 ± 0.7 | 2 (3) | 3:1 | ||
12 h | 6, 7 | 8 | Migration of epithelial cells | 5.1 ± 0.6 | 5 (4–6) | 1.6 ± 0.5 | 2 (1–2) | 3:1 | |
1 day | 8, 9 | 8 | 5.4 ± 1.2 | 6 (3–6) | 2.9 ± 1.0 | 2.5 (2–4) | 2:1 | ||
2 days | 10–13 | 4 | 8 | Angiogenesis | 4.8 ± 1.6 | 6 (2–6) | 2.3 ± 0.5 | 2 (2–3) | 2:1 |
3 days | 14, 15 | 4 | Fibroblasts | 3.8 ± 0.5 | 4 (3–4) | 3.5 ± 0.6 | 3.5 (3–4) | 1:1 | |
4 days | 12, 13, 16, 17 | 4 | 4 | Granulation tissue | 3 ± 1.4 | 3 (1–5) | 2.9 ± 0.6 | 3 (2–4) | 1:1 |
5 days | 18,19 | 4 | 2 ± 0.8 | 2 (1–3) | 3.3 ± 0.5 | 3 (3–4) | 1:2 | ||
6 days | 14, 15 | 4 | 2.3 ± 1.0 | 2.5 (1–3) | 3.3 ± 0.5 | 3 (3–4) | 1:1 | ||
7 days | 20, 21 | 4 | 1.8 ± 0.5 | 2 (1–2) | 3.8 ± 0.5 | 4 (3–4) | 1:2 | ||
8 days | 16, 17 | 4 | Hemosiderophages | 2.8 ± 1.0 | 2.5 (2–4) | 3.8 ± 0.5 | 4 (3–4) | 1:1 | |
10 days | 18, 19 | 4 | 2.8 ± 2.1 | 2.5 (1–5) | 3.8 ± 1.0 | 3.5 (3–5) | 1:1 | ||
14 days | 22, 23 | 4 | 1 ± 0 | 1 (1–1) | 2 ± 0 | 2 (2–2) | 1:2 | ||
18 days | 20, 21, 24, 25 | 4 | 4 | Complete epithelization | 1.1 ± 1.0 | 1 (0–3) | 2 ± 0.5 | 2 (1–3) | 1:2 |
27 days | 22, 23 | 4 | 0 | 0 | 2 ± 0 | 2 (2–2) | – | ||
35 days | 24, 25 | 4 | 0 | 0 | 1.5 ± 0.6 | 1.5 (1–2) | – |