Quantitative immunoassay for mink immunoglobulin in serum and milk

Table 2 Intra- and inter-assay variation for both serum and milk samples

	n	Serum		%CV	n	Milk		%CV
		OD_{450−650 nm}				OD_{450−650 nm}
		Mean	SD			Mean	SD
Intra-assay variation	12	1.97	0.04	2.15	12	2.15	0.06	2.71
	12	1.42	0.03	2.41	12	1.63	0.05	3.27
	12	0.84	0.02	2.44	12	1.05	0.04	3.51
	12	0.54	0.02	3.71	12	0.66	0.02	3.35
	12	0.37	0.01	3.79	12	0.40	0.01	2.91
	12	0.27	0.02	5.97	12	0.28	0.01	4.15
	12	0.23	0.01	4.48	12	0.22	0.01	5.92
Inter-assay variation	2	2.40	0.35	14.75	3	2.09	0.09	4.20
	2	1.80	0.32	17.78	3	1.58	0.09	5.55
	2	1.01	0.14	14.12	3	1.04	0.09	8.43
	2	0.62	0.07	10.98	3	0.64	0.05	7.94
	2	0.41	0.03	7.68	3	0.36	0.05	12.99
	2	0.29	0.02	6.22	3	0.25	0.03	12.14
	2	0.24	0.01	5.17	3	0.19	0.03	16.03

The intra-assay variation of the serum and milk IgG sandwich ELISA was determined using 12 replicates of seven dilutions of the sample (n = 12). Inter-assay variation was determined by performing 12 replicates of seven dilutions of either one serum sample on five different plates measured on 2 different days (n = 2) or one milk sample on two different plates measured on 3 different days (n = 3). SD standard deviation, CV coefficient of variation

ISSN: 1751-0147