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Table 2 Intra- and inter-assay variation for both serum and milk samples

From: Quantitative immunoassay for mink immunoglobulin in serum and milk

  n Serum %CV n Milk %CV
OD450−650 nm OD450−650 nm
Mean SD Mean SD
Intra-assay variation 12 1.97 0.04 2.15 12 2.15 0.06 2.71
  12 1.42 0.03 2.41 12 1.63 0.05 3.27
  12 0.84 0.02 2.44 12 1.05 0.04 3.51
  12 0.54 0.02 3.71 12 0.66 0.02 3.35
  12 0.37 0.01 3.79 12 0.40 0.01 2.91
  12 0.27 0.02 5.97 12 0.28 0.01 4.15
  12 0.23 0.01 4.48 12 0.22 0.01 5.92
Inter-assay variation 2 2.40 0.35 14.75 3 2.09 0.09 4.20
  2 1.80 0.32 17.78 3 1.58 0.09 5.55
  2 1.01 0.14 14.12 3 1.04 0.09 8.43
  2 0.62 0.07 10.98 3 0.64 0.05 7.94
  2 0.41 0.03 7.68 3 0.36 0.05 12.99
  2 0.29 0.02 6.22 3 0.25 0.03 12.14
  2 0.24 0.01 5.17 3 0.19 0.03 16.03
  1. The intra-assay variation of the serum and milk IgG sandwich ELISA was determined using 12 replicates of seven dilutions of the sample (n = 12). Inter-assay variation was determined by performing 12 replicates of seven dilutions of either one serum sample on five different plates measured on 2 different days (n = 2) or one milk sample on two different plates measured on 3 different days (n = 3). SD standard deviation, CV coefficient of variation