Fig. 2

Flow cytometry analysis by Ann V/PI assay for apoptosis. Chondrocytes were cultured for 24 h in medium with 10% FBS (FBS-cells) or in medium with 10% PRP (PRP-cells) and then treated with 1% LD, 1% LD + 10% PRP or 10%PBS. a Representative dot plots of the two experimental groups, are shown. Different subpopulations were classified in necrotic cells (V−/PI+), late apoptotic cells (V+/PI+), live cells (V−/PI−) and early apoptotic cells (V+/PI−). b Bar graphs represent the percentage (mean ± SD) of apoptotic cells (early + late apoptotic cells) in each condition. The experiments were repeated thrice, in triplicates *P < 0.05 1% LD vs PBS in each experimental group; **P < 0.001 1% LD vs 1% LD + 10% PRP in each experimental group