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Fig. 4 | Acta Veterinaria Scandinavica

Fig. 4

From: The in vitro effect of progesterone on the orexin system in porcine uterine tissues during early pregnancy

Fig. 4

The influence of P4 (10, 100, 1000 nM) on orexin type 1 receptor (OX1R) mRNA (a) and protein (b) expression in the porcine myometrium on days 10 to 11, 12 to 13, 15 to 16 and 27 to 28 of pregnancy, and on days 10 to 11 of the oestrous cycle. The gene expression was determined by quantitative real-time PCR (a). The protein concentration was determined by the western blotting analysis (b); upper panels: representative immunoblots (MM—molecular marker); lower panels: densitometric analysis of OX1R protein relative to actin protein. Results are reported as the mean ± SEM (n = 5). Bars with different superscripts differ (P < 0.05)

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