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Table 1 Characteristics of primers used in the study of gene expression in porcine endometrial and myometrial explants

From: The in vitro effect of progesterone on the orexin system in porcine uterine tissues during early pregnancy

Gene Primers sequences Accession number Complementary position Primer, nM References
PPO F: 5′-AAGACGACACCCTTCCTGGAGAC-3′ EF434655 F: 41–63 500 [9]
R: 5′-TGATTGCCAGCGCCGTGTAGCA-3′ R: 240–261 500
OX1R F: 5′-ACCGCTGGTATGCCATCTACCAC-3′ AF097995 F: 27–49 500 [5]
R: 5′-ATAGAGGTCATCTGCCCAGCGTTCA-3′ R: 208–232 500
OX2R F: 5′-GACATCACGGAGACCTGGTTCTTC-3′ AF059740 F: 30–53 500 [5]
R: 5′-GATGTAGGAGACGATCCAGATGATGAC-3′ R: 207–233 500
PPIA F: 5′-GCACTGGTGGCAAGTCCAT-3′ AY266299 F: 219–237 300 [42]
R: 5′-AGGACCCGTATGCTTCAGGA-3′ R: 269–299 300
ACTB F: 5′-ACATCAAGGAGAAGCTCTGCTACG-3′ U07786 F: 266–289 500 [43]
R: 5′-GAGGGGCGATGATCTTGATCTTCA-3′ R: 608–631 500
  1. PPO prepro-orexin, OX1R orexin type 1 receptor, OX2R orexin type 2 receptor, PPIA cyclophilin, ACTB β-actin; F forward, R reverse