Table 1 Characteristics of primers used in the study of gene expression in porcine endometrial and myometrial explants
Gene | Primers sequences | Accession number | Complementary position | Primer, nM | References |
|---|---|---|---|---|---|
PPO | F: 5′-AAGACGACACCCTTCCTGGAGAC-3′ | EF434655 | F: 41–63 | 500 | [9] |
R: 5′-TGATTGCCAGCGCCGTGTAGCA-3′ | R: 240–261 | 500 | |||
OX1R | F: 5′-ACCGCTGGTATGCCATCTACCAC-3′ | AF097995 | F: 27–49 | 500 | [5] |
R: 5′-ATAGAGGTCATCTGCCCAGCGTTCA-3′ | R: 208–232 | 500 | |||
OX2R | F: 5′-GACATCACGGAGACCTGGTTCTTC-3′ | AF059740 | F: 30–53 | 500 | [5] |
R: 5′-GATGTAGGAGACGATCCAGATGATGAC-3′ | R: 207–233 | 500 | |||
PPIA | F: 5′-GCACTGGTGGCAAGTCCAT-3′ | AY266299 | F: 219–237 | 300 | [42] |
R: 5′-AGGACCCGTATGCTTCAGGA-3′ | R: 269–299 | 300 | |||
ACTB | F: 5′-ACATCAAGGAGAAGCTCTGCTACG-3′ | U07786 | F: 266–289 | 500 | [43] |
R: 5′-GAGGGGCGATGATCTTGATCTTCA-3′ | R: 608–631 | 500 |