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Table 1 Lectin-histochemical staining with biotinylated STL and Cy5-streptavidin combined with immunofluorescence double labelling of alpaca forebrain tissue sections

From: Neuropathological findings suggestive for a stroke in an alpaca (Vicugna pacos)

First primary antibodies

First visualising immunoreagents

Second primary antibodies

Second visualising immunoreagents

Rabbit-anti-Iba (1:400; Synaptic Systems, Göttingen, Germany)

Cy3-donkey-anti-rabbit IgG

Guinea pig-anti-GFAP (1:200; Synaptic Systems)

Cy2-donkey-anti-guinea pig IgG

Rabbit-anti-AQP4 (1:200; Alomone, Jerusalem, Israel)

Cy3-donkey-anti-rabbit IgG

Guinea pig-anti-GFAP (1:200; Synaptic Systems)

Cy2-donkey-anti-guinea pig IgG

Rabbit-anti-Iba (1:400; Synaptic Systems)

Cy3-donkey-antirabbit IgG

Guinea pig-anti-NeuN (1:200, Synaptic Systems)

Cy2-donkey-anti-guinea pig IgG

Rabbit-anti-NF-L (1:200; Synaptic Systems)

Cy3-donkey-anti-rabbit IgG

Goat-anti-collagen IV (1:50; Merck Millipore, Billerica, MA, USA)

Cy2-donkey-anti-goat IgG

  1. Biotinylated STL was obtained from Vector Laboratories (Burlingame, CA, USA) and applied at 20 µg/mL for 20 h. All fluorescent immunoreagents were supplied by Dianova (Hamburg, Germany) and used at 20 µg/mL for 1 h
  2. STL Solanum tuberosum agglutinin (= potato lectin), Iba ionized calcium binding adapter molecule-1, GFAP glial fibrillary acidic protein, AQP4 aquaporin-4, NeuN neuronal nuclei, NF-L neurofilament, light chain
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Acta Veterinaria Scandinavica

ISSN: 1751-0147