Table 1 Comparison between automatic and manual assays for estimation of ceruloplasmin
From: Validation of an automated assay for measurement of bovine plasma ceruloplasmin
Parameters | Automatic assay | Manual assay |
|---|---|---|
Number and path of cuvette | One cuvette with ½ cm optical path | Two cuvettes with 1 cm optical path |
Amount of the sample | 20 µL | 100 µL |
Amount of sodium acetate buffer | 180 µL | 2000 µL |
Amount of p-phenylenediamine buffer | 90 µL | 1000 µL |
Amount of sodium azide 1.5 mol/L | NA | 50 µL |
Reaction time (consumed time till obtaining the results) | 20.8 min | 30 min |