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Table 1 Comparison between automatic and manual assays for estimation of ceruloplasmin

From: Validation of an automated assay for measurement of bovine plasma ceruloplasmin

Parameters Automatic assay Manual assay
Number and path of cuvette One cuvette with ½ cm optical path Two cuvettes with 1 cm optical path
Amount of the sample 20 µL 100 µL
Amount of sodium acetate buffer 180 µL 2000 µL
Amount of p-phenylenediamine buffer 90 µL 1000 µL
Amount of sodium azide 1.5 mol/L NA 50 µL
Reaction time (consumed time till obtaining the results) 20.8 min 30 min
  1. NA not applicable