First cases of contagious ovine digital dermatitis in Germany

Table 1 Findings in claw swabs or claw biopsies by different bacteriological methods

Animal no.	Sampling at	TWDS-Score	Detection of Spirochaetes by Gram stain	D. nodosus culture	D. nodosus PCR	Treponema spp. PCR	T. medium/T. vincentii-like	T. phagedenis-like	T. pedis
1	Clinic	15	+	n.d.	–	+	+	+	+
2	Clinic	18	+	n.d.	±	+	+	+	+
3	Farm	34	n.d.	–	+	+	+	+	+
4	Farm	72	n.d.	–	+	+	+	+	+
5	Farm	102	n.d.	–	±	+	+	+	+
6	Farm	52	n.d.	–	±	+	+	+	+
7	Farm	36	n.d.	–	±	n.d.	n.d.	n.d.	n.d.
8	Farm	40	n.d.	–	+	+	+	+	+
9	Farm	4	n.d.	–	+	+	+	+	+
10	Farm	34	n.d.	+	+	+	+	+	+
11	Farm	136	n.d.	+	+	+	+	+	+
12	Farm	68	n.d.	–	+	+	–	+	+
13	Control	0	n.d.	n.d.	n.d.	–	–	–	–
14	Control	0	n.d.	n.d.	n.d.	+	+	–	+
15	Control	0	n.d.	n.d.	n.d.	+	–	–	–
16	Control	0	n.d.	n.d.	n.d.	+	–	–	–
17	Control	0	n.d.	n.d.	n.d.	–	–	–	–
18	Control	0	n.d.	n.d.	n.d.	+	–	–	–

+, positive; - , negative; ±, inconclusive; TWDS, Total weighted digital score according to Wittington and Nicholls (1995) [41]; n.d., not determined

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