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Table 1 Findings in claw swabs or claw biopsies by different bacteriological methods

From: First cases of contagious ovine digital dermatitis in Germany

Animal no. Sampling at TWDS-Score Detection of Spirochaetes by Gram stain D. nodosus culture D. nodosus PCR Treponema spp. PCR T. medium/T. vincentii-like T. phagedenis-like T. pedis
1 Clinic 15 + n.d. + + + +
2 Clinic 18 + n.d. ± + + + +
3 Farm 34 n.d. + + + + +
4 Farm 72 n.d. + + + + +
5 Farm 102 n.d. ± + + + +
6 Farm 52 n.d. ± + + + +
7 Farm 36 n.d. ± n.d. n.d. n.d. n.d.
8 Farm 40 n.d. + + + + +
9 Farm 4 n.d. + + + + +
10 Farm 34 n.d. + + + + + +
11 Farm 136 n.d. + + + + + +
12 Farm 68 n.d. + + + +
13 Control 0 n.d. n.d. n.d.
14 Control 0 n.d. n.d. n.d. + + +
15 Control 0 n.d. n.d. n.d. +
16 Control 0 n.d. n.d. n.d. +
17 Control 0 n.d. n.d. n.d.
18 Control 0 n.d. n.d. n.d. +
  1. +, positive; - , negative; ±, inconclusive; TWDS, Total weighted digital score according to Wittington and Nicholls (1995) [41]; n.d., not determined