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Table 2 Checkerboard titrations

From: Development and evaluation of indirect enzyme-linked immunosorbent assays for the determination of immune response to multiple clostridial antigens in vaccinated captive bred southern white rhinoceros (Ceratotherium simum simum)

ELISA antigen

Antigen coating dilution

Serum dilution

C. perfringens type A

1/3000

1/800

C. chauvoei

1/800

1/800

C. septicum

1/160

1/100

C. sordellii

1/800

1/1600

C. novyi

1/3200

1/200

  1. Summary of the checkerboard titrations for the individual iELISAs showing the optimum antigen dilution for coating the plates as well as the serum dilution that falls in the middle of the titration curve
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Acta Veterinaria Scandinavica

ISSN: 1751-0147