Table 1 Cytochemical stains used to identify the lineage of myeloid cells [6, 46]
From: A review of current knowledge of myeloproliferative disorders in the horse
Cytochemical reaction stain | Cellular element stained | Blasts/cell type identified |
|---|---|---|
Myeloperoxidase (MPO) | Neutrophil primary granules | Myeloblasts strong + /monoblasts faint + Neutrophil; Monocyte |
Sudan Blanck B (SBB) | Phospholipids | Myeloblasts strong + /monoblasts faint + Neutrophil; Monocyte |
Naphtol AS-D chloroacetate esterase (CAE); specific | Esterese enzymes | Myeloblasts strong + Granulocytes |
Leukocyte alkaline phosphatase (ALP) | Neutrophil secondary granules | Metamyelocytes, band and segmented neutrophil Monocyte |
Alpha naphthyl acetate esterase (ANAE); non-specific Alpha naphthyl butyrate esterase (ANBE); non-specific | Esterase enzymes | Monoblasts strong + Monocyte strong + |