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Table 1 Cytochemical stains used to identify the lineage of myeloid cells [6, 46]

From: A review of current knowledge of myeloproliferative disorders in the horse

Cytochemical reaction stain Cellular element stained Blasts/cell type identified
Myeloperoxidase (MPO) Neutrophil primary granules Myeloblasts strong + /monoblasts faint + 
Neutrophil; Monocyte
Sudan Blanck B (SBB) Phospholipids Myeloblasts strong + /monoblasts faint + 
Neutrophil; Monocyte
Naphtol AS-D chloroacetate esterase (CAE); specific Esterese enzymes Myeloblasts strong + 
Granulocytes
Leukocyte alkaline phosphatase (ALP) Neutrophil secondary granules Metamyelocytes, band and segmented neutrophil
Monocyte
Alpha naphthyl acetate esterase (ANAE); non-specific
Alpha naphthyl butyrate esterase (ANBE); non-specific
Esterase enzymes Monoblasts strong + 
Monocyte strong + 
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