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Table 3 PCR results in cycle threshold (Ct) values of tissue samples from goats inoculated with a Vietnamese goatpox virus isolate (n = 8) and controls (n = 2)

From: Experimental infection of indigenous North African goats with goatpox virus

Goat No.

Group 1a

Group 2a

Group 3a

Control

335

129

373

269

287

261

265

752

759

267

Tissue

Ct value

 Subcutaneous tissue at injection site

19.4

18.2

18.9

20.2

20.5

NS

NS

NS

NS

NS

 Skin crusts

16.6

16.6

16.5

18.3

14.9

14.6

17.1

17.3

NS

NS

 Secondary nodules

NS

NS

NS

20.8

22.1

22.6

24.7

31.4

NS

NS

 Testis

NS

19.1

NS

31.4

NS

NS

NS

NS

NS

NS

 Trachea

22.9

25.6

NS

23.5

32.8

30.8

36.4

36.3

–

–

 Lung

–

–

–

36.2

–

–

35.7

–

–

–

 Pulmonary lymph node

29.9

–

36.2

33.7

–

–

31.8

–

–

–

 Heart

–

–

34.9

34.1

NS

NS

NS

NS

–

–

 Spleen

–

–

NS

32.1

34.4

–

–

–

–

–

 Liver

–

–

NS

–

–

–

35.1

–

–

–

 Rumen

NS

NS

NS

29.8

NS

NS

NS

NS

NS

NS

 Abomasum

37.2

–

NS

35.1

NS

NS

NS

NS

NS

NS

 Reticulum

30.8

NS

NS

29.3

NS

NS

NS

NS

NS

NS

 Omasum

24.3

35.9

NS

29.8

NS

NS

NS

NS

NS

NS

 Duodenum

–

–

NS

33.9

–

NS

NS

NS

NS

NS

 Mesenteric lymph node

34.4

–

NS

NS

34.1

36.5

36.7

34.7

–

–

 Kidney

34.7

–

NS

–

–

–

–

–

–

–

 Blood

–

–

–

–

–

–

–

–

–

–

  1. Tissues were sampled at day 16 (group 1) post infection (pi) or day 18 pi (group 2, group 3, Control)
  2. NS not sampled
  3. –: Negative, i.e. below detection level
  4. aGroup 1, Group 2, Group 3: Inoculated goats were retrospectively divided into 3 groups, based on severity of clinical signs at the end of the experiment