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Table 3 Multivariate analysis for semen quality characteristics of fresh and frozen-thawed semen and elements Fe, Se and Zn measured in seminal plasma on the day of collection

From: Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

Variables

Elements in seminal plasma (mg/L)

Fe

Se

Zn

Semen characteristics (%)

β (95% CI)

P

β (95% CI)

P

β (95% CI)

P

Fresh semen

 Motilitya

1,30,610 (38,101–2,23,118)

0.0075

−1,26,431 (−1,164,930 to 9,12,069)

0.8044

61,517 (−58,790 to 1,81,823)

0.3029

 Progressive motilitya

1,19,776 (56,976–1,82,575)

0.0006

−77,622 (−7,82,609 to 6,27,364)

0.8227

42,553 (−39,118–1,24,223)

0.2940

 Acrosome defectsb

−0.4749 (−0.8642 to −0.0855)

0.0188

2.3681 (−2.0026 to 6.7388)

0.2756

−2.0780 (−1.0140 to −0.0013)

0.0494

 DNA fragmentation

−1.6851 (−3.1603 to −0.2099)

0.0268

−1.9652 (−18.5260 to 14.5956)

0.8092

−1.3558 (−3.2743 to 0.5627)

0.1583

Frozen-thawed semen

 Viabilitya

1,14,891 (24,909–2,04,872)

0.0143

−1,56,982 (−1,167,111 to 8,53,146)

0.7519

72,103 (−44,917 to 1,89,124)

0.2165

 DNA fragmentation

−3.4984 (−5.7577 to −1.2391)

0.0038

−5.7146 (−31.0774 to 19.6481)

0.6471

−1.0197 (−3.9579 to 1.9185)

0.4820

  1. aCubic transformation
  2. bLog transformation