Fig. 3
From: Centrifugal force assessment in ram sperm: identifying species-specific impact
Effect on ram sperm functionality induced by three centrifugal forces. Ram sperm a viability (Zombie Violet™) (%), b caspases 3 and 7 activation (CellEvent™ Caspase-3/7 Green) (%), and c high mitochondrial activity (CellROX™ Deep Red) (%). The same 11–8 males were analyzed in each experimental group (600×g, 3000×g, and 6000×g for 10 min at room temperature) at 0 h and 2 h post-incubation at 37 °C. Graph dots represent individual male ejaculates. Different lowercase superscripts letters (a, b) indicate significant differences (P ≤ 0.05) for each assessment point among the centrifugal forces. FlowJo analyses for d viability (Zombie Violet™), e apoptosis (CellEvent™ Caspase-3/7 Green), and f high mitochondrial functionality (CellROX™ Deep Red). Ram sperm confocal images of Control samples labelling for g plasma membrane (Zombie Violet™), h DNA (CellEvent™ Caspase-3/7 Green), and i intracellular ROS (CellROX™ Deep Red) employed in the flow cytometry analyses