Table 1 Antibodies used in this study and related features
From: Undifferentiated laryngeal carcinoma with hyaline bodies in a cat
Target | Marker | Clone | Dilution | Secondary antibody | Manufacturer |
|---|---|---|---|---|---|
Epithelial markers | panCK | AE1/AE3 | 1:100 | Mouse | Dakocytomation |
CK8/18 | 5D3 | 1:30 | Mouse | Novocastra | |
CK5/6 | D5/I6 B4 | 1:50 | Mouse | Invitrogen | |
Myoepithelial markers | p63 | 4A4 | 1:200 | Mouse | Santa Cruz Biotechnology, Inc |
Calponin | Calp | 1:200 | Mouse | Dakocytomation | |
Mesenchymal marker | Vimentin | V9 | 1:150 | Mouse | Dakocytomation |
(Neuro)endocrine markers | Neurofilament | 2F11 | 1:100 | Mouse | Invitrogen |
Chromogranin | NS55 | 1:50 | Rabbit | Dakocytomation | |
Lymphoid markers | CD3 | F7238 | 1:50 | Mouse | Dakocytomation |
CD20 | L6 | 1:800 | Mouse | Leica Biosystems |