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Table 1 The coefficient of variation (CV) from an initial faecal sample slurry, fresh or frozen, for three different dilutions (1; 1:1; 1:9) for: (a) NucleoSpin DNA Stool, (b) E.Z.N.A. Stool DNA Kit, and (c) QIAmp Fast DNA Stool Mini Kit

From: Assessment of three DNA extraction kits for the absolute quantification of strongyle nematode eggs in faecal samples

Sample

n

Ratio faecal slurry:water

Coefficient of variation

NucleoSpin DNA Stool

E.Z.N.A. Stool DNA Kit

QIAmp Fast DNA Stool Mini Kit

Fresh

5

1

27.5

91.0

102.8

Fresh

5

1:1

31.6

108.8

100.9

Fresh

5

1:9

71.2

78.6

223.6

Frozen

5

1

13.1

96.5

77.1

Frozen

5

1:1

45.1

52.8

133.8

Frozen

5

1:9

87.1

181.3

158.8