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Table 2 The arithmetic mean and standard deviation of DNA yield from an initial faecal slurry, fresh or frozen, for three different dilutions (1; 1:1; 1:9) for: (a) NucleoSpin DNA Stool, (b) E.Z.N.A. Stool DNA Kit, and (c) QIAmp Fast DNA Stool Mini Kit

From: Assessment of three DNA extraction kits for the absolute quantification of strongyle nematode eggs in faecal samples

 

DNA yield

Ratio faecal slurry:water

1

1:1

1:9

NucleoSpin DNA Stool

 Fresh

141,040 ± 38,726

46,084 ± 14,581 (↓)

10,140 ± 7216 (↓)

 Frozen

82,960 ± 10,844

30,228 ± 13,634 (↓)

12,652 ± 11,021 (↑)

E.Z.N.A. Stool DNA Kit

 Fresh

9188 ± 8363

2387 ± 2595 (↓)

176 ± 139 (↓)

 Frozen

915 ± 883

284 ± 150 (↓)

6 ± 11 (↓)

QIAmp Fast DNA Stool Mini Kit

 Fresh

22 ± 22

6 ± 6 (↓)

0.3 ± 0.6 (↓)

 Frozen

1079 ± 832

290 ± 388 (↓)

8 ± 12 (↓)

  1. The arrows indicate how the expected DNA yield corresponds to the DNA yield in the undiluted sample