Skip to main content

Table 2 Results for 88 methicillin-resistant Staphylococcus aureus (MRSA) strains spa typed by polymerase chain reaction (PCR) and/or whole genome sequencing (WGS)

From: Low-level colonization of methicillin-resistant Staphylococcus aureus in pigs is maintained by slowly evolving, closely related strains in Finnish pig farms

Number of strains

spa type (PCR)

spa type (WGS)a

Sequence type (ST)a, b

Resistance genesa

Virulence genesa, c

SCCmec elementa

Study A

      

Farm 1

Farm 2

      

 13

1

t034

t011

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 2

0

t034

t011

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 1

0

t034

t1255

ST398

mecA, blaZ, str, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 8

1

t034

t034

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 1

0

t034

t034

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 2

1

Failed

t011

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 0

3

Failed / Not sequenced

t034

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 1

0

t034

Unknown

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 7

1

t034

Not whole genome sequenced / sequencing failed

 1

0

t1255

Not whole genome sequenced

Study B

   

Farm 1

Controlled facility

  

 0

21

t034

t034

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 9

0

Not sequenced

t034

ST398

mecA, blaZ, lnu(B), lsa(E), tet(M), tet(K), dfrG

aur, hlgA, hlgB, hlgC

Vc (5C2&5)

 0

15

t034

Not whole genome sequenced

  1. aThe following tools from the Center for Genomic Epidemiology were used: spaTyper 1.0 [35] for spa typing, MLST [37] for multilocus sequence typing, ResFinder [38] for resistance gene detection, VirulenceFinder [39] for virulence gene detection and SCCmecFinder 1.2 [36] for staphylococcal cassette chromosome mec (SCCmec) typing
  2. bMultilocus sequence type
  3. cVirulenceFinder database for MRSA includes virulence genes hlb, hlgABC, tst, lukED, lukFS-PV, etAB, edinABC, aur, splABE, scn, sak, ACME and enterotoxins A-E, G-O, R, U, Q