Table 1 Enterococcus faecium virulence gene primer sequence and product length
Target genes | Primer sequence (5′–3′) | Product size (bp) | Ta ( °C) |
|---|---|---|---|
cylA | ACTCGGGGATTGATAGGC GCTGCTAAAGCTGCGCTT | 688 | 56 |
cpd | TGGTGGGTTATTTTTCAATTC TACGGCTCTGGCTTACTA | 782 | 50 |
asa1 | GCACGCTATTACGAACTATGA TAAGAAAGAACATCACCACGA | 375 | 51 |
ace | GGAATGACCGAGAACGATGGC GCTTGATGTTGGCCTGCTTCCG | 616 | 51 |
acm | GGCCAGAAACGTAACCGATA CGCTGGGGAAATCTTGTAAA | 353 | 51 |
esp | AGATTTCATCTTTGATTCTTGG AATTGATTCTTTAGCATCTGG | 510 | 56 |
gelE | AATTGCTTTACACGGAACGG GAGCCATGGTTTCTGGTTGT | 548 | 51 |
hyl | ACAGAAGAGCTGCAGGAAATG GACTGACGTCCAAGTTTCCAA | 276 | 56 |