Table 3 Drug-resistant gene of polymerase chain reaction (PCR) procedure
Target genes | Ta ( °C) | PCR procedure |
|---|---|---|
blaTEM | 49 | 95 °C 2 min + {95 °C 1 min + 49 °C 1 min + 72 °C 1 min} [47] × 35 + 72 °C 7 min |
blaSHV | 53 | 95 °C 3 min + {95 °C 1 min + 53 °C 1 min + 72 °C 1 min} × 35 + 72 °C 7 min |
blaCTX-M | 51 | 94 °C 2 min + {94 °C 30 s + 51 °C 30 s + 72 °C 30 s} × 35 + 72 °C 3 min |
optrA | 50 | |
cfr | 56 | 95 °C 5 min + {94 °C 30 s + 1 min + 72 °C 1 min} × 30 + 72 °C 5 min |
poxtA | 58 | |
aac(6')-aph(2'') | 61 | |
aph(2'')-Ib | 55 | 94 °C 10 min + {94 °C 1 min + 1 min + 72 °C 1 min} × 30 + 72 °C 10 min |
aph(2'')-Ic | 55 | |
aph(2'')-Id | 53.4 | |
vanA | 54 | 94 °C 2 min + {94 °C 1 min + 54 °C 1 min + 72 °C 1 min} × 30 + 72 °C 10 min |
vanB | 54 | |
ermA | 52 | 93 ℃ 3 min + {93 °C 1 min + 52 °C 1 min + 72 °C 1 min} × 35 + 72 °C 5 min |
ermB | 52 |