Antimicrobial resistance in Staphylococcus spp., Escherichia coli and Enterococcus spp. in dogs given antibiotics for chronic dermatological disorders, compared with non-treated control dogs
© The Author(s) 2002
Received: 01 November 2003
Accepted: 17 November 2003
Published: 31 March 2004
The aim of this study was to evaluate antimicrobial resistance in canine staphylococci, Escherichia coli and enterococci, which were isolated from 22 dogs with pyoderma and a history of previous antibiotic treatment, compared to bacterial isolates from 56 non-treated control dogs. Two isolates of each bacterial species per dog were investigated, if detected. Staphylococcal isolates from dogs with pyoderma (35 isolates) were more resistant to sulphatrimethoprim than the isolates from controls (56 isolates) (57% vs. 25%, p < 0.004). Multiresistance in staphylococci was also more common in dogs with pyoderma (29% vs. 9%, p = 0.02). A similar trend among isolates of E. coli was detected (24 and 74 isolates from treated and control dogs, respectively), but the differences were not significant. Resistance for macrolide-lincosamides was approximately 20% among staphylococci in both groups. Resistance to ampicillin among enterococci was 4%–7%. The age of the dogs might have an impact on resistance: multiresistance among staphylococcal isolates from younger dogs (≤5 years) was more common than in older dogs (≥6 years) (24%, vs. 0%, 63 and 27 isolates, respectively, p = 0.02). Staphylococci in younger dogs were more resistant to tetracycline (48% vs. 11%, p < 0.001) and sulphatrimethoprim (48% vs. 15%, p < 0.01) than those in older dogs. In contrast, the isolates of E. coli from older dogs tended to be more resistant, although a significant difference was detected only in resistance to tetracycline (13% vs. 2% of 40 and 50 isolates respecthely, p = 0.04)). The results of this small study indicate that resistance in canine staphylococci in the capital area of Finland is comparable with many other countries in Europe. Resistance in indicator bacteria, E. coli and enterococci, was low.
Keywordsantimicrobial resistance normal flora Staphylococcus spp. Enterococcus faecalis Enterococcus faecium Escherichia coli pyoderma dog.
Antibiotika resistens hos Staphylococcus spp., Escherichia coli och Enteroccus spp. isolerade från hundar med kroniska dermatologiska sjukdomar, i förhållande till friska hundar.
Målet med denna studie var att jämföra stafylokockers, E. colis och enterokockers antibiotikaresistens hos hundar med pyoderma (n=22), vilka tidigare behandlats med antibiotika, med antibiotikaresistensen hos icke-behandlade kontrollhundar (n=56). Resistens mot sulfatrimetoprim var mer allmänn hos stafylokocker från hundar med pyoderma (35 isolat) än stafylokocker från kontrollhundar (56 isolat) (57% vs. 25%, p< 0.004). Stafylokocker var mera multiresistenta hos hundar med pyoderma (29% vs. 9%, p=0.02). En liknande trend kunde konstateras även hos E. coli (24 isolat från pyoderma och 74 isolat från kontrolhundar), men skillnaden var inte signifikant. Ampicillin resistens var 4%-7% hos Enterococcus spp. isolat.
Hundens ålder kan ha betydelse för resistensen - multiresistenta stafylokocker var vanligare hos yngre hundar (≤5 år) än hos äldre (≥6 år) (24% vs. 0%, n=63 respektive 27, p=0.02). Stafylokocker isolerade från yngre hundar var oftare resistenta mot tetracyklin och sulfatrimetoprim. Däremot var E. coli från äldre hundar mera resistenta, men en signifikant skillnad kunde konstateras enbart mot tetracyklin (12.5% vs. 1.7%, n=40 respektive 58, p=0.04). Resultaten från denna lilla studie visar att resistensen hos stafylokocker hos hundar i Finland är jämförbar med resistensen i många andra länder i Europa. Resistensen hos E. coli och enterokocker verkar däremot vara låg i Finland.
Keywordsantimicrobial resistance normal flora Staphylococcus spp. Enterococcus faecalis Enterococcus faecium Escherichia coli pyoderma dog.
Staphylococcus intermedius, as well as other staphylococci, is habitant of the normal bacterial flora of the dog and can be isolated from healthy dogs, especially from the anal region . S. intermedius is also an important skin pathogen in dogs . Antimicrobial resistance among canine staphylococci is common [19, 13, 27, 9]. In addition, bacteria of the normal flora of the gut, such as Escherichia coli and Enterococcus spp., can easily acquire and transfer resistance genes. These bacteria can thus be used as indicators of changes in antimicrobial resistance . Regular monitoring of the level of resistance in pathogens and bacteria of the normal flora has been recommended . Monitoring programmes for antimicrobial resistance have been established in veterinary medicine, but only for food producing animals . Not many reports of the level of antimicrobial resistance in canine normal flora have been published [8, 17, 5, 26]. The aim of this study was to investigate whether the bacteria of the normal flora are more resistant in dogs, which have received antimicrobials for the treatment of chronic dermatological disorders, when compared to the bacteria of non-treated control dogs. We chose to study staphylococci, E. coli, Enterococcus faecalis and Enterococcus faecium.
Materials and methods
Dogs with chronic dermatological disorders (n = 22) treated with antimicrobials during the 6 months preceding the study were sampled in the treated group. The last treatment was to have ended at least 2 weeks before the samples were taken. Dogs in the control group (n = 56) were presented to a veterinarian for other reasons and had not received antimicrobials during the previous 6 months. The following data were collected from the patients: breed, age, sex, antimicrobials given during the previous 6 months and the duration of the treatment.
Samples were collected at the Small Animal Veterinary Teaching Hospital of the Helsinki University and at 2 veterinary clinics in the same area between December 1997 and July 1998. For the isolation of staphylococci, a sample was taken by swabbing the perianal mucosal area with a sterile cotton swab. For the isolation of E. coli and enterococci, a faecal sample was taken from the rectum with a sterile glove. Samples were directly transported to the National Veterinary and Food Research Institute, Helsinki, Finland, where the bacteriological analysis was carried out immediately after sample arrival. If samples were taken during a weekend, they were first stored at +4°C and then transported to be analysed on Monday.
Isolation and identification of bacteria
For the isolation of staphylococci, samples were streaked onto 5% bovine blood agar and Staphylococcus medium 110 -agar (Difco Laboratories, Detroit, Michigan, USA) and incubated at 37°C for 18–24 h. Identification to the species level was performed by conventional methods .
For the isolation of E. coli, faecal samples were cultured on Rapid E. coli -agar (Sanofi Diagnostics, Marnes La Coquette, France) and incubated at +44°C for 24 h. Five blue colonies were picked, streaked onto blood agar and incubated further at +37°C for 24 h. E. coli was identified by colony morphology, oxidase and indole tests. The identity was further confirmed with API20E (bioMérieux, Marcy l'Etoile, France).
For the isolation of enterococci, faecal samples were inoculated onto Slanetz-Bartley agar (Merck, Darmstadt, Germany) and incubated at +44°C for 48 h. Five red colonies were subcultured on Tryptone soya agar (BBL, Cockeysville, Maryland, USA) and incubated at 37°C for 24 h. Isolates were identified as enterococci on the basis of colony morphology on Slanetz-Bartley agar, a negative catalase test and the ability to grow in Brain Heart Infusion (BHI) (Difco Laboratories) containing 6.5% NaCl and at pH 9.6. E. faecium and E. faecalis were identified on the basis of tests for motility, fermenting reactions with L-arabinose, mannitol, melibiose, raffinose and sorbitol. The identification of isolates was further confirmed with APIStrept (bioMérieux, Marcy l'Etoile, France). If Staphylococcus spp., E. coli, E. faecium and/or E. faecalis were identified, 2 colonies per sample were stored in BHI supplemented with glycerol at -70°C.
Antimicrobial susceptibility testing
Susceptibility breakpoint zone diameters (mm) used in the study.
Respective MIC -value for susceptible srains *
Penicillin G 10 IU
Ampicillin 10 μg
Amoxillin-clavulanate (2:1) 30 μg
Cephalotin 30 μg
Cefotaxime 30 μg
Oxacillin 1 μg
Macrolides and lincosamides
Erythromycin 15 μg
Clindamycin 2 μg
Streptomycin 10 μg
Gentamicin 10 μg
Chloramphenicol 30 μg
Enrofloxacin 5 μg
Trimethoprim/sulfamethoxazole 1.25/23.75 mg (SXT)
Tetracycline 30 μg
for E.coli: ≤4 μg/ml
Vancomycin 30 μg
Five staphylococcal isolates, which first gave an unusual macrolide resistance pattern (isolates resistant or intermediate to clindamycin but sensitive to erythromycin) were re-tested both with E-test (AB-Biodisk, Solna, Sweden) and disk diffusion test. The susceptibility breakpoint in E-test both to erythromycin and clin-damycin was MIC ≤0.5 μg/ml. The phenotypes of these isolates were tested with double-disk method . Enterococcal isolates, which had reduced susceptibility to vancomycin according to the disk diffusion test, were tested on the presence of van -genes with a multiplex PCR (polymerase chain reaction) detection at a National Public Health Institute with the method described by .
The results are presented as the number of bacterial isolates. Fisher's 2-tailed exact test was used to compare resistance in bacterial isolates between the 2 groups. The statistical analysis was made with the SAS software. Difference between the groups was considered significant if p ≤ 0.05.
A total of 56 dogs, with a median age of 5 years, were included in the control group, and 22 dogs, with a median age of 3 years in the treated group. Of the treated dogs, 9 had received antimicrobials during at least 2 treatment periods lasting more than 3 weeks, and the rest of the dogs were given antibiotics for less than 3 weeks. The number of treatment periods with antimicrobials in dermatological patients was: cephalexin 14, amoxicillin-clavulanate 4, amoxicillin 3, penicillin V 2, sulphatrimethoprim (SXT) 1 and clindamycin 1. Information on the antimicrobial agent used was missing for 4 dogs with pyoderma. The number of investigated bacterial isolates in the control and treated group, respectively, was as follows: Staphylococcus spp. 56/35, E. coli 74/24, E. faecalis 28/21 and E. faecium 45/16. The respective mean number of bacterial isolates investigated per dog was: Staphylococcus spp. 1.8/1.8, E. coli 1.9/1.8, E. faecalis 1.6/1.9 and E. faecium 1.9/1.8. The number in parentheses (n) hereafter is the number of bacterial isolates, if not stated otherwise.
Among enterococci, the resistance to ampicillin was low in both groups (4%–7%) and there was no difference between the groups. Two E. faecalis isolates from 2 control dogs were classified as resistant to vancomycin, but no van -genes were present in multiplex PCR testing. None of E. faecium isolates were resistant to vancomycin.
The impact of the age of the dog on the results was further studied by dividing the dogs into 2 age groups (≤0–5 years, and ≥6 years) regardless of the previous treatment history. Multiresistant staphylococcal isolates were more commonly found in younger than older dogs (24%, n = 63 vs. 0%, n = 27, p = 0.02) Staphylococcal isolates from younger dogs were also more resistant to tetracycline (48% vs. 11%, p < 0.001) and SXT (48% vs. 15%, p < 0.01, Fig. 1). E. coli isolates showed the opposite pattern in resistance: isolates from older dogs tended to be more resistant compared with isolates from younger dogs, but a significant difference was detected only in the resistance to tetracycline (13%, n = 40 vs. 2%, n = 58, p = 0.04).
The findings of this study indicate that antimicrobial resistance in canine staphylococci is at a similar level in the capital area of Finland compared with studies from Norway , Sweden , France , Denmark , USA, UK and Germany . Betalactamase production among canine S. intermedius has been reported to be common, 50%–90% of isolates produce betalactamase [19, 22, 11, 13, 9].
In spite of an extensive use of first generation cephalosporins and other β-lactamase stable antimicrobials in the canine practice, resistance to oxacillin is still rather rare [22, 11, 13], although methicillin resistance in canine staphylococci has been reported to occur [24, 6, 21]. It should also be noted that the routine disk diffusion test is not optimal for detecting methicillin resistance . In our study, resistance to first generation cephalosporins or oxacillin was not detected.
The level of SXT resistance among canine S. intermedius varies in different countries. In our study, SXT resistance was more common in staphylococci isolated from treated dogs (57%) compared to controls (25%).  reported similar resistance figures to SXT among S. intermedius isolates from healthy dogs in France. In the same study, however, resistance among S. intermedius isolated from dogs with pyoderma increased from 6% to 36% during a 9-year follow-up period. In contrast, in the UK, resistance to SXT in staphylococci from canine pyoderma cases peaked at 15% in 1989, but fell to 8% by 1995 . Interestingly, in Norway and in Denmark, despite of extensive use of SXT products in canine practice, resistance to this agent was very low (1% and 0%) in 1996 [22, 11]. Due to the molecular genetics of SXT resistance, removal of the selection pressure will not have an immediate impact on the level of resistance .
The relatively high macrolide-lincosamide resistance (on average 20%) in canine staphylococci in this study might be explained by an increased use of these antimicrobials in dogs during the last decades in our country. Similarly,  reported from 3% to 25% increase in resistance to macrolide-lincosamide antimicrobials during 1987–1994 in canine staphylococci in Norway, which was probably due to an increased use of these drugs. In France, macrolide-lincosamide resistance in canine staphylococci was as high as 40% . Usually, as a rule, bacteria which are resistant to clindamycin are also resistant to macrolides. In susceptibility testing they thus show MLSB phenotype (resistance to macrolide-lincosamide-streptogramin-B antimicrobials), which is mediated by erm -methylase genes . In the study by  it was reported that all erythromycin resistant canine S. intermedius strains carried erm(B)-gene. Interestingly,  described one human strain of Streptococcus pyogenes, which showed resistance to clindamycin (≥MIC 2 μg/ml) but not to erythromycin in MIC-testing. Since the strain showed resistance to azithromycin, they still considered it to be of the MLSB -phenotype. However, this strain did not carry any erm -genes, but instead a point mutation in 23S rRNA was found. Since the number of reports from macrolide resistance mechanisms in canine gram-positive bacteria is scarce, more studies will be needed.
Faecal gram-negative bacteria and enterococci are considered to be good indicators of the selection pressure caused by the use of antimicrobials . In our study, the level of resistance was low in canine enterococci and E. coli isolates.  investigated E. coli strains from healthy urban and rural dogs and observed more multiresistance in E. coli isolates from rural dogs living on dairy farms than in E. coli isolates from urban dogs. These dogs had not received antimicrobials, but the selection pressure was supposed to be derived from antimicrobials used for cows.  reported that 69% of E. coli strains isolated from canine urinary infections were resistant to at least 2 antimicrobials, and multiresistance was also common among patients who had not received antimicrobials before. The incidence of R-plasmids has been shown to be common in the faecal flora of healthy dogs 
Resistance to ampicillin in enterococci was low in our study, and no differences were detected between the groups. Two (2/49) E. faecalis isolates had reduced susceptibility to vancomycin according to disk diffusion test, but these isolates did not carry any van -genes and thus were not real VRE isolates (=vancomycin resistant enterococcus). None of the 61 E. faecium isolates was resistant to vancomycin. Vancomycin resistant enterococci in dogs have been reported in other studies, probably due to avoparcin use in farm animals . The VRE prevalence in enterococci from Dutch dogs was 48%, and half of these carried an identical van(A)-gene found also in human VRE -strains . In Belgium, 8% of canine enterococci were resistant to vancomycin and also carried the van(A)-gene . The use of avoparcin was banned in Finland in 1995, and the use of vancomycin for the treatment of animals is legally prohibited.
According to our results, the effect of age on resistance would need more studies, although material in this study was too small to do any further conclusions. In humans,  reported increased resistance among urinary E. coli isolates with increasing age, but they were not able to conclude if this was due to the ageing or the cumulative lifetime use of antimicrobials. We were not able to find reports where the effect of age on resistance had been studied in dogs.
In conclusion, our results support the fact that the use of antimicrobials, and the development of and prevailing antimicrobial resistance among bacteria are linked together. Although resistance among indicator bacteria was found to be low, resistance in staphylococci to commonly used antimicrobials is widespread: the efficacy of sulphatrimethoprim and macrolides is at risk to deteriorate. The rather high prevalence of multiresistant strains among canine staphylococci is also a phenomenon, which gives cause for concern. In order to detect early changes in bacterial susceptibilities before a high prevalence of resistance is selected or developed, regular monitoring of antimicrobial resistance both among pathogenic bacteria and normal flora of companion animals will be needed. The genetic mechanisms, which mediate antimicrobial resistance in these bacteria, would also need further studies.
The authors thank Dr. Jaana Vuopio-Varkila from the National Public Health Institute, the Department of Microbiology, for the screening of van -genes from the enterococcal isolates.
- Arstila T, Huovinen S, Lager K, Lehtonen A, Huovinen P: Positive correlation between the age of patients and the degree of antimicrobial resistance among urinary strains of Escherichia coli. J Infect. 1994, 29: 9-16. 10.1016/S0163-4453(94)94925-5.View ArticlePubMedGoogle Scholar
- Boerlin P, Burnens AP, Frey J, Kuhnert P, Nicolet J: Molecular epidemiology and genetic linkage of macrolide and aminoglycoside resistance in Staphylococcus intermedius of canine origin. Vet Microbiol. 2001, 79: 155-169. 10.1016/S0378-1135(00)00347-3.View ArticlePubMedGoogle Scholar
- Caprioli A, Busani L, Martel JL, Helmuth R: Monitoring of antibiotic resistance in bacteria of animal origin: epidemiological and microbiological methodologies. Int J Antimicrob Agents. 2000, 14: 295-301. 10.1016/S0924-8579(00)00140-0.View ArticlePubMedGoogle Scholar
- Devriese LA, De Pelsmaecker K: The anal region as a main carrier site of Staphylococcus intermedius and Streptococcus canis in dogs. Vet Rec. 1987, 121: 302-303.View ArticlePubMedGoogle Scholar
- Devriese LA, Ieven M, Goossens H, Vandamme P, Pot B, Hommez J, Haesebrouck F: Presence of vancomycin-resistant enterococci in farm and pet animals. Antimicrob Agents Chemother. 1996, 40: 2285-2287.PubMed CentralPubMedGoogle Scholar
- Gortel K, Campbell KL, Kakoma I, Whittem T, Schaeffer DJ, Weisiger RM: Methicillin resistance among staphylococci isolated form dogs. Am J Vet Res. 1999, 60: 1526-1530.PubMedGoogle Scholar
- Hirsh DC: Multiple antimicrobial resistance in Escherichia coli isolated from urine of dogs and cats with cystitis. JAVMA. 1973, 162: 885-887.PubMedGoogle Scholar
- Hirsh DC, Ling GV, Ruby AL: Incidence of R-plasmids in fecal flora of healthy household dogs. Antimicrob Agents Chemother. 1980, 17: 313-315.PubMed CentralView ArticlePubMedGoogle Scholar
- Holm BR, Petersson U, Mörner A, Bergström K, Franklin A, Greko C: Antimicrobial resistance in staphylococci from canine pyoderma: a prospective study of first-time and recurrent cases in Sweden. Vet Rec. 2002, 151: 600-605.View ArticlePubMedGoogle Scholar
- Huovinen P, Sundström L, Swedberg G, Sköld O: Minireview: trimethoprim and sulfonamide resistance. Antimicrob Agents Chemother. 1995, 39: 279-289.PubMed CentralView ArticlePubMedGoogle Scholar
- Kruse H, Hofshagen M, Thoresen SI, Bredal WP, Vollset I, Søli NE: The antimicrobial susceptibility of Staphylococcus species isolated from canine dermatitis. Vet Res Com. 1996, 20: 205-214. 10.1007/BF00366918.View ArticleGoogle Scholar
- Leclercq R, Courvalin P: Bacterial resistance to macrolide, lincosamide and streptogramin antibiotics by target modification. Antimicr Agents Chemother. 1991, 35: 1267-1272.View ArticleGoogle Scholar
- Lloyd DH, Lamport AI, Feeney C: Sensitivity to antibiotics amongst cutaneous and mucosal isolates of canine pathogenic staphylococci in the UK, 1980–1996. Vet Dermatol. 1996, 7: 171-175.View ArticleGoogle Scholar
- Malbruny B, Nagai K, Coguemont M, Bozdogan B, Andrasevic AT, Hupkova H, Leclercq R, Appelbaum PC: Resistance to macrolides in clinical isolates of Streptococcus pyogenes due to ribosomal mutations. J Antimicrob Chemother. 2002, 49: 935-939. 10.1093/jac/dkf038.View ArticlePubMedGoogle Scholar
- Martel J-L, Tardy F, Sanders P, Boisseau J: Review: New trends in regulatory rules and surveillance of antimicrobial resistance in bacteria of animal origin. Vet Res. 2001, 32: 381-392. 10.1051/vetres:2001131.View ArticlePubMedGoogle Scholar
- Medleau L, Long RE, Brown J, Miller WH: Frequency and antimicrobial susceptibility of Staphylococcus species isolated from canine pyoderma. Am J Vet Res. 1986, 47: 229-231.PubMedGoogle Scholar
- Monaghan C, Tierney U, Colleran E: Antibiotic resistance and R-factors in the fecal coliform flora of urban and rural dogs. Antimicrob Agents Chemother. 1981, 19: 266-270.PubMed CentralView ArticlePubMedGoogle Scholar
- National Committee for Clinical Laboratory Standards (NCCLS): Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; tentative standard, NCCLS document M31-A-1997, Villanova, Pa, United States.
- Noble WC, Kent LE: Antibiotic resistance in Staphylococcus intermedius isolated from cases of pyoderma in the dog. Vet Dermatol. 1992, 3: 71-74.View ArticleGoogle Scholar
- Patel RJ, Uhl R, Kohner P, Hopkins MK, Cockerill FR: Multiplex PCR detection of vanA, vanB, vanC-1, and van C2/3 genes in enterococci. J Clin Microbiol. 1997, 35: 703-707.PubMed CentralPubMedGoogle Scholar
- Pak IS, Han HR, Shimizu A: Characterization of methicillin-resistant Staphylococcus aureus isolated from dogs in Korea. J Vet Med Sci. 1999, 61: 1013-1018. 10.1292/jvms.61.1013.View ArticlePubMedGoogle Scholar
- Pedersen K, Wegener HC: Antimicrobial susceptibility and rRNA gene restriction patterns among Staphylococcus intermedius from healthy dogs and from dogs suffering from pyoderma or otitis externa. Acta vet Scand. 1995, 36: 335-342.PubMedGoogle Scholar
- Pellerin JL, Bourdeau P, Sebbag H, Person JM: Epidemiosurveillance of antimicrobial compound resistance of Staphylococcus intermedius clinical isolates from canine pyodermas. Comp Immunol Microbiol Infect Dis. 1998, 21: 115-133. 10.1016/S0147-9571(97)00026-X.View ArticlePubMedGoogle Scholar
- Piriz S, Valle J, Mateos EM, De La Fuente R, Cid C, Ruiz-Santaquiteria JA, Vadillo S: In vitro activity of fifteen antimicrobial agents in methicillin-resistant and methicillin-susceptible Staphylococcus intermedius. J Vet Pharmacol Ther. 1996, 19: 118-123.View ArticlePubMedGoogle Scholar
- Quinn PJ, Carter ME, Markey B, Carter GR: Clinical Veterinary Microbiology. 1994, Wolfe, London, UKGoogle Scholar
- Van Belkum A, van den Braak N, Thomassen R, Verbrugh H, Endtz H: Vancomycin-resistant enterococci in cats and dogs. Letter to the Editor Lancet. 1996, 348: 1038-1039.Google Scholar
- Werckenthin C, Cardoso M, Martel J-L, Schwarz S: Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus and canine Staphylococcus intermedius. Review article Vet Res. 2001, 32: 341-362. 10.1051/vetres:2001129.View ArticlePubMedGoogle Scholar