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  • Open Access

Comparison of Different Methods for Measuring Immunoglobulin Content in Calf Serum

  • 1 and
  • 1
Acta Veterinaria Scandinavica200344(Suppl 1):P26

https://doi.org/10.1186/1751-0147-44-S1-P26

Published: 31 March 2003

Keywords

  • Serum Total Protein
  • IgG1 Concentration
  • Passive Transfer
  • IgG1 ELISA
  • Serum IgG1 Concentration

Objectives

The correlation between an IgG1 ELISA and different methods for immunoglobulin and serum protein determination was examined in calves 1–77 days of age to compare methods to establish failure of passive transfer of immunoglobulins.

Materials and methods

Blood samples were taken from 92 calves and age, sex, general condition and weight (weighing or thorax measure) were recorded. In serum total protein concentration was measured by Biuret's method and refractometer (Atago, Bie and Berntsen A/S). Immunoglobulin contents was masured by the semiquantitative serum glutaraldehyde test (Tennant et al. 1979, 174, 848–853, a commercial whole blood IgG test (Quick Test Calf Whole Blood IgG K™, Midland Bio-Products Corporation) and a direct sandwich IgG1 ELISA developed in our laboratory.

Results

Significant correlations was found between serum IgG1 concentration and serum protein concentration measured by refractometer or Biuret and between the two methods of protein determination. Tests positive (>10 g/l IgG) with the commercial test kit had significant higher IgG1 concentration (29.79 g/l) than negative test (9.75 g/l). Significant correlation was also demonstrated between IgG1 and the glutaraldehyde coagulation test.

The table below shows the sensitivity, specificity, positive (posPV) and negative (negPV) predictive value and % correct classified calves (CCC) with the commercial test kit (CTK), the glutaraldehyde test (GAT) and refractometer (REFR) in relation to an IgG1 >10 g/l = no failure of passive transfer and IgG1 <10 g/l = failure of passive transfer.

Table 1

Method

sensitivity

specificity

posPV

negPV

%CCC

Commercial

     

test kit

0.70

0.92

0.88

0.79

81.57

GAT time

     

   10 min

0.35

0.02

0.24

0.03

17.36

   15 min

0.58

1

1

0.73

80.59

   20 min

0.58

0

0.24

0

27.13

REFR

     

   40 g/l

0.05

1

0

0.54

55.49

   50 g/l

0.54

0.90

0.82

0.69

72.85

   55 g/l

0.95

0.69

0.73

0.94

81.83

   60 g/l

1

0.45

0.61

1

70.63

Conclusion

With ELISA IgG1 as reference significant correlations were demonstrated between the various methods used. In calves from 1–77 days of age a serum protein concentration of 55 g/l measured by refractometer correctly classified 81.63% of the calves as having serum IgG1 concentrations below 10 g/l. Therefore, the simple refractometer method is recommended for field use to measure failure of colostrum uptake in individual calves.

Authors’ Affiliations

(1)
Internal Medicine, Department of Clinical Studies, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark

Copyright

© The Author(s); licensee BioMed Central Ltd. 2003

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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