Figure 1

The microarray contained 720 unique bovine cDNAs arrayed in 48 patches across a glass microscope slide (a), each gene was spotted in triplicate within a single patch (b). Each patch was designed as an 8 × 8 array of spotted cDNAs originating from our bovine total leukocyte (BOTL) library (see [74]; this issue). Multiple controls were included in each patch as shown in panel (b): 3 GAPDH spots (yellow arrow heads), 2 synthetic lambda Q gene spots (white arrow heads) with one in the upper right corner of each patch surrounded by 3 blank spots (white box), 16 blank spots (black areas), and 1 spot containing only DMSO (white circle). In addition, 14 unique BOTL cDNAs spotted in triplicate were included per patch (e.g., red and green arrow heads in panel b).