Figure 4

Regression analysis of the BOTL test spots on the cDNA microarray shown in Fig. 1. In this analyses, Cy3 spot intensities (volumes) were regressed on Cy5 spot intensities to determine if the leukocytes collected 6 hours postpartum (h +6) had different gene expressions than leukocytes collected 14 days prepartum (d -14). This analysis showed that most leukocyte genes represented on our microarray were equally expressed on d -14 and h +6 because the majority of the spots on the scatter plot clung tightly to the regression line, which had a slope very close to 1.0. However, this analysis revealed 54 overlapping spots in the Cy5 area of the plot, which represented triplicate spots for 18 unique BOTL cDNAs that were repressed 6 hours after parturition.