Figure 9

Northern blot validation of repressed gene expression in leukocytes from parturient dairy cows. Three BOTL clones that appeared to have triplicate spots of true red the cDNA microarray (see Fig. 1b for an example) were selected for use as cDNA probes on Northern blot analyses of leukocyte RNA from 4 mid-lactation control cows (RNA was pooled; gray bars) and from one periparturient cow collected 14 days prepartum (d -14; open bars) and 6 hours postpartum (h +6; striped bars). Band intensities for the 3 test transcripts were normalized against band intensities for GAPDH. These data confirm dye intensity results in Fig. 5 and 6, dye ratio results in Fig. 8, and visual microarray results in Fig. 1, that genes represented by BOTL clones 6XG09R, 8_F09, and 13_B06 are repressed right after parturition.