An Immunogenomics Approach to Understanding Periparturient Immunosuppression and Mastitis Susceptibility in Dairy Cows*

Table 1 Results of BLASTN analysis of 18 BOTL sequences shown by cDNA microarray analysis to be repressed by parturition.

BOTL Clone Name	P-value ¹⁾ (d-14 vs h +6)	BLASTN results (putative gene identification)	E-value ²⁾	TIGR Cluster (TC#) or GenBank # ³⁾
Observed by microarray analysis and confirmed by Northern blot hybridization:
6XGO9R	0.02	Kruppel-like factor mRNA	10^-37.69	TC71106
8_F09	0.04	Mitogen activated protein kinase kinase kinase 10 (MAP3K10) mRNA	10–57.15	4505262 11425967
13_B06	0.04	Unknown	-	-
Observed by microarray analysis:
9_G07	0.06	Unknown	-	-
5XC11R	0.13	Bos taurus lysosomal alpha-mannosidase (ManB) gene	10^-9	TC74217
4XD09R	0.002	Unknown	-	-
8_B07	0.004	Basic leucine zipper transcription factor-like mRNA	10^-116	TC68695
12_B09	0.002	Bovine beta-globulin gene	10^-36.3	TC61928
12_B06	0.001	Bovine ornithine decarboxylase (ODC) gene	10^-19.52	TC69145
8_B05	0.02	Unknown	-	-
6XC10R	0.01	Mus musculus plectin isoform plec 0a, 1c (Plec 1) mRNA	10^-28	6578732
4XD08R	0.06	Bos taurus matrix acidic phosphoprotein mRNA	10^-10	TC76407
10_A07	0.007	Unknown	-	-
3XA10R	0.01	Diphosphoinositol polyphosphate phosphohydrolase	10^-32.15	TC69522
6XH09R	0.003	Long chain fatty acid CoA ligase 4 (acyl CoA synthetase 4)	10^-126	TC72044
3XD01R	0.004	Diphosphoinositol polyphosphate phosphohydrolase mRNA	10–31.52	TC69522
8_F08	0.0002	Unknown	-	-
2XH12R	0.0006	Bovine mRNA for MHC class II DM β-chain	10^-41.69	TC6553

¹⁾ P-value from the t-tests comparing sample time dye intensities of BOTL spots on the cDNA microarray,
²⁾ expectation values (E-value) from the BLAST search, and
³⁾ TIGR cluster number or GenBank number to which the BOTL sequence had homology.

ISSN: 1751-0147