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Figure 3 | Acta Veterinaria Scandinavica

Figure 3

From: Commercially Available Antibodies to Human Tumour Necrosis Factor-α Tested for Cross-Reactivity with Ovine and Bovine Tumour Necrosis Factor-α using Flow Cytometric Assays

Figure 3

Mean fluorescence intensity from 3 bovine serum samples containing 14000, 15400 and 15600 pg TNF-α/ml, respectively, and 2 pooled serum samples not containing any TNF-α (0 pg/ml), as previously determined with ELISA. Concentrations of bovine TNF-α in the samples were determined with Fluorokine® MAP-kit, before (black bar) and after (grey bar) addition of 50 μl 2 ng/ml recombinant human TNF-α. The dashed line marks the fluorescence of microspheres incubated with buffer only, whereas the solid line marks the fluorescence of microspheres incubated with buffer containing 50 μl 2 ng/ml recombinant human TNF-α.

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Acta Veterinaria Scandinavica

ISSN: 1751-0147