Table 1 DPX RT-PCR results of lymphoid tissue analyses from groups 1, 2 and the negative control group (Ctr), vaccinated at one, three and three week of age, respectively, and challenged with field virus at four weeks. The chickens were sampled in groups of three.
Age in days | Days after challenge | Group | Chicken No. | RT-PCR Bursa of Fabricius | RT-PCR Bone marrow | RT-PCR Thymus | RT-PCR Bursa swabs |
|---|---|---|---|---|---|---|---|
28 | 0 | 1 | 1,2,3 | A A A | 0 0 0 | 0 0 0 | ND |
- | 2 | 1,2,3 | A A A | A 0 0 | 0 0 0 | 0 A A | |
29 | 1 | 1 | 4,5,6 | A 0 0 | 0 0 0 | 0 0 0 | ND |
- | 2 | 4,5,6 | A AB A | 0 0 0 | 0 0 0 | A A 0 | |
30 | Ctr | 4,5,6 | A A A | 0 0 0 | 0 0 0 | ND | |
2 | 1 | 7,8,9 | A A 0 | 0 0 0 | 0 0 0 | ND | |
- | 2 | 7,8,9 | A A A | 0 0 0 | 0 0 0 | 0 A 0 | |
31 | 3 | 1 | 10,11,12 | A A A | 0 0 0 | 0 0 0 | ND |
- | 2 | 10,11,12 | A A AB | 0 0 0 | 0 0 B | 0 0 B | |
36 | 8 | 1 | 13,14,15 | 0 0 0 | 0 0 0 | 0 0 0 | ND |
- | 2 | 13,14,15 | A A A | 0 0 A | 0 0 0 | 0 0 0 | |
38 | Ctr. | 7,8,9 | 0 0 A | 0 0 0 | 0 0 0 | ND | |
10 | 1 | 16,17,18 | 0 0 A | 0 0 0 | 0 0 0 | ND | |
- | 2 | 16,17,18 | A A A | 0 0 0 | 0 0 0 | 0 0 0 | |
42 | 14 | 1 | 19,20,21 | 0 0 0 | 0 A 0 | 0 0 0 | ND |
- | 2 | 19,20,21 | A 0 A | 0 0 0 | 0 0 0 | A 0 0 | |
44 | 16 | 1 | 22,23,24 | 0 0 0 | B 0 0 | 0 0 0 | ND |
- | 2 | 22,23,24 | A A AB | 0 0 0 | 0 0 0 | 0 0 0 |