Figure 1

Multiplex PCR with primers crp-1, crp-2 and IS10as2. A fragment of 273 base pairs was produced inside the crp gene from the wild type Salmonella enterica serotype Gallinarum biovar gallinarum G9 (lanes 1 and 4) and crp⁺ from pSD110 in the re-complemented strain (lane 3 and 6). A fragment of 500 base pairs was amplified between crp-1 and one of the IS sequences in Tn10 in the mutant (lane 2 and lane 5) and the re-complemented strain (lanes 3 and 6).