Acta Veterinaria Scandinavica

Table 1 Results of mapping by PCR and analysis of expression of down stream genes by RT-PCR in Salmonella enterica serotype Gallinarum biovar gallinarum (G9, J91) and Salmonella enterica serotype Gallinarum biovar pullorum (3).

From: Effects of crp deletion in Salmonella enterica serotype Gallinarum

Strains	Genotype	cpr- PCR	Tn10-PCR	argD-PCR	cysG- PCR	yfhk- RT- PCR	argD RT-PCR	cysG RT-PCR
G9	Wt	+	-	+	+	+	+	+
G9,	Δcrp	-	+	-	-	-	-	-
G9	Δcrp + pSD110	+	+	-	-	-	-	-
J91	Wt	+	-	+	+	+	+	+
J91	Δcrp	-	+	-	-	-	-	-
J91	Δcrp + pSD110	+	+	-	-	-	-	-
3	Wt	+	-	+	+	+	+	+
3	Δcrp	-	+	-	-	-	-	-
3	Δcrp + pSD110	+	+	-	-	-	-	-

(+) – PCR reactions producing amplicons of the size expected; (-) no amplicon obtained with the primers used; Wt – Wild type; (Details of primers and PCR conditions used are detailed in Table 2)

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ISSN: 1751-0147

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