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Table 2 PCR primers and conditions used to characterize Δcrp mutants of Salmonella enterica serotype Gallinarum biovar gallinarum (G9) and Salmonella enterica serotype Gallinarum biovar pullorum (3).

From: Effects of crp deletion in Salmonella enterica serotype Gallinarum

Gene

Primer

Sequence (5'→3')

Gene accession number used for primer design

PCR conditions

crp

crp-1

GGTGCTTGGCAAACCGC

M13773 &M13770

1

 

crp-2

GCGGTTTTCGCACGTACC

  

Tn10

crp-1

GGTGCTTGGCAAACCGC

 

1

 

IS10as2

CGTTAAGCTGTTGAGTCG

AY583239

 

argD

argD-1

CGGCAGAGTTTATTCCGG

AE008859

2

 

argD-2

CCATACCGCGAATATCGC

  

cysG

cysG-1

CGACTGTCTGATCGTCGG

AE008859

2

 

cysG-2

CCTTTCAGGCGTACCACG

  
 

cysG-3

CCATGTAGAACACCAGCG

  

yhfK

Yhfk1

CACTACGGCAAACGCTGGTG

AE008859

3

 

Yhf2

AGCAGGCTGTATTTCGCTTC

  

argD

argD-3

GAACCATGCGAACCTACATG

AE008859

3

 

argD-4

TGATGAGGTGATTCTGCCTG

  

cysG

cysG-4

AAACGCTTCTCGACTCGTGT

AE008859

3

 

cysG-5

TCATAATGTCGTCGGAGACG

  
  1. 1 – 94°C/5'; 94°C/30'; 60°C/1'; 72°C/2'; 72°C/10' (30 cycles)
  2. 2 – 94°C/5'; 94°C/30';58°C/1'; 72°C/2'; 72°C/10' (30 cycles)
  3. 3 – RT-PCR according to Sleator et al. [13].
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Acta Veterinaria Scandinavica

ISSN: 1751-0147