Table 1 Sequences of primers used for genotyping of the mice (to detect the 466J14 BAC clone containing human CYP2C18 and CYP2C19)
Primer | Primer sequence (5'-3') | PCR product length (bp) |
|---|---|---|
BAC5'endF | TAACATTAGCAGGTGAAGCCCAAA | 706 |
BAC5'endR | CAATCTGTTCCATGATGGTTGATG | |
BAC3'endF | AGACTGTGCTATCATGGGAACCAA | 480 |
BAC3'endR | GTTTTCTTGGGCTGAATGTCCTCT | |
2C18intron6F | GGCAAGAAACACTTCATGAGCACT | 429 |
2C18intron6R | ATTCAGTTAAGGCCTCCCTTTTCC | |
2C19intron5F | CAAGATGGGCCTTATAAAGTTGGC | 727 |
2C19intron5R | GAAGAAATTGGAACCCTCATGTCC |