Dendrogram showing UPGMA clustering of PhenePlate typing data for vancomycin resistant E. faecium , E. hirae and E. durans isolated from sludge (S). For comparison, vancomycin resistant E. faecium from sewage, chickens and humans are included. The horizontal axis of the dendrogram shows the similarities between isolates, and the dotted line indicates the identity level of 0.975. PFGE types are indicated for isolates tested by pulsed field gel electrophoresis (PFGE). Filled circles indicate isolates with vanA, the other isolates carried the vanB gene. Arrows indicate the isolates from direct culture.