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Table 1 Primer -and plasmid sequences for qPCR of the A. phagocytophilum msp2 (p44) expression site (partial sequence).

From: Variant -and individual dependent nature of persistent Anaplasma phagocytophilum infection

Primer

Sequence

Conc.

Tm °C

ApMSP2252

5'ACAGTCCAGCGTTTAGCAAGA-3'

0.5 μ M

57.0

ApMSP2459

5'GCACCACCAATACCATAACCA-3'

0.5 μ M

56.5

Plasmid construct

5'GAATTCGCCCTTACAGTCCAGCGTTTAGCAAGA TAAGAGATTTTAGTA

TAAGGGAGAGTAACGGAGAGACTAAGGCAGTATATCCATACTTAAAGGA

TGGAAAGAGTGTAAAGCTTGAGTCTAACAAGTTTGACTGGAACACTCCTG

ATCCTCGGATTGGGTTTAAGGACAACATGCTTGTAGCTATGGAAGGCAGT

GTTGGTTATGGTATTGGTGGTGC CAGGGTTGAGCTTGAGATTGGTTACG

AGCGCTTCAAGACCAAAAGGGCGAATTCT-3'

  1. Primer binding sites are indicated (underscored) in the sequence of the plasmid construct. The concentrations of primers are calculated for the final reaction mix.
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Acta Veterinaria Scandinavica

ISSN: 1751-0147