Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice

Table 2 The antigens described and vaccine components of immunized mice

Groups	vaccine components
	antigens described	protein content
Control	total protein concentration was 100 μg/ml	rApxI, rApxII, rApxIII and rOMP
Group I	total protein concentration was 150 μg/ml	rApxI, rApxII, rApxIII, rApxIV, rOMP and rApfa
Group II	10⁹ colony forming units (CFU)/ml	inactivated APP1 whole cell
Group III	phosphate-buffered saline (PBS)	PBS

Note:
1. APP1 inactivated with 0.3% formaldehyde solution.
2. The vaccine components of all the immunized groups as well as the control group were emulsified with an equal volume of mineral oil adjuvant (Sigma-Aldrich).
Effect of challenge with APP1 and APP2 on vaccinated mice

ISSN: 1751-0147