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Table 2 The antigens described and vaccine components of immunized mice

From: Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice

Groups vaccine components
  antigens described protein content
Control total protein concentration was 100 μg/ml rApxI, rApxII, rApxIII and rOMP
Group I total protein concentration was 150 μg/ml rApxI, rApxII, rApxIII, rApxIV, rOMP and rApfa
Group II 109 colony forming units (CFU)/ml inactivated APP1 whole cell
Group III phosphate-buffered saline (PBS) PBS
  1. Note:
  2. 1. APP1 inactivated with 0.3% formaldehyde solution.
  3. 2. The vaccine components of all the immunized groups as well as the control group were emulsified with an equal volume of mineral oil adjuvant (Sigma-Aldrich).
  4. Effect of challenge with APP1 and APP2 on vaccinated mice