Table 3 Recovery of 12 ng/mL of bPL added to five foetal samples (A to E) in the presence of different dilutions of rabbit anti-bPL.
Initial serum: bPL (ng/mL) | Identification of foetal sample | Foetal bPL concentrations (ng mL-1) and percentage of recovery | |||||
|---|---|---|---|---|---|---|---|
Rabbit anti-bPL dilutions | |||||||
1:1,280,000 | 1:320,000 | 1:80,000 | 1:20,000 | 1:5,000 | 1:2,500 | ||
A: 8.9 ± 1.6 | A+ | 8.6 | 7.5 | 3.7 | 2.6 | 1.6 | 0.7 |
A* | 20.6 | 19.5 | 15.7 | 14.6 | 13.6 | 12.7 | |
A++ | 20.5 | 19.0 | 13.9 | 11.3 | 6.9 | 5.4 | |
Recovery (%) | 99.4 | 97.4 | 88.3 | 77.3 | 50.4 | 42.9 | |
B: 10.0 ± 1.3 | B+ | 11.5 | 7.6 | 6.3 | 6.0 | 1.5 | 0.7 |
B* | 23.5 | 19.6 | 18.3 | 18.0 | 13.5 | 12.7 | |
B++ | 23.9 | 18.4 | 17.2 | 14.9 | 8.1 | 6.3 | |
Recovery (%) | 101.7 | 93.9 | 93.9 | 82.7 | 60.2 | 49.1 | |
C: 17.5 ± 1.4 | C+ | 18.1 | 19.4 | 15.4 | 16.4 | 3.3 | 0.9 |
C* | 30.1 | 31.4 | 27.4 | 28.4 | 15.3 | 12.9 | |
C++ | 29.5 | 28.5 | 23.8 | 20.7 | 10.7 | 5.3 | |
Recovery (%) | 98.1 | 90.8 | 87.1 | 72.8 | 70.1 | 41.4 | |
D: 18.5 ± 1.9 | D+ | 20.3 | 21.0 | 14.6 | 12.4 | 6.6 | 0.9 |
D* | 32.3 | 33.0 | 26.6 | 24.4 | 18.6 | 12.9 | |
D++ | 31.5 | 30.9 | 24.4 | 19.5 | 10.5 | 5.9 | |
Recovery (%) | 97.3 | 93.7 | 91.6 | 80.0 | 56.5 | 45.7 | |
E: 21.3 ± 1.5 | E+ | 22.56 | 19.3 | 21.2 | 15.3 | 5.2 | 1.2 |
E* | 34.56 | 31.3 | 33.2 | 27.3 | 17.2 | 13.2 | |
E++ | 35.60 | 28.8 | 30.6 | 20.2 | 11.2 | 6.3 | |
Recovery (%) | 103.01 | 92.2 | 92.2 | 74.1 | 64.8 | 47.7 | |