Table 1 Recommendations for testing dogs from areas endemic for B. canis when mated or inseminated with dogs from non-endemic areas, or when imported to non-endemic areas

1

Mating or insemination, no suspicion of B. canis and no reproductive disturbances

Blood sample for antibody analysis

2

Mating or insemination with dog with previous reproductive disturbances, import of dogs without reproductive disturbances.

Serology: 2 samples 4-6 weeks apart

3

Import of or mating/insemination with dogs with previous reproductive disturbances, infection with B. canis suspected.

Serology, 2 samples 4-6 weeks apart, blood culture and culture or PCR from semen or vagina

4

Import of or mating/insemination with dog from kennel with endemic infection of B. canis.

Dissuaded from mating or import.